abstract：:The goal of incorporating renewable carbon into the fuel and chemical enterprise will most likely be successful when combined systems of biocatalysts and chemical catalysts are exploited. Significant efforts in the biocatalytic release of sugars from biomass are being pursued for subsequent use in fermentation. Two re...

journal_title：ACS chemical biology

authors： Shanks BH

更新日期：2007-08-17 00:00:00

abstract：:KCNE transmembrane peptides are a family of modulatory beta-subunits that assemble with voltage-gated K+ channels, producing the diversity of potassium currents needed for proper function in a variety of tissues. Although all five KCNE transcripts have been found in cardiac and other tissues, it is unclear whether two...

journal_title：ACS chemical biology

authors： Morin TJ,Kobertz WR

更新日期：2007-07-20 00:00:00

abstract：:Conotoxins are small disulfide-rich peptides from the venom of cone snails. Along with other conopeptides, they target a wide range of membrane receptors, ion channels, and transporters, and because of their high potency and selectivity for defined subtypes of these receptors, they have attracted a great deal of atten...

journal_title：ACS chemical biology

authors： Craik DJ,Adams DJ

更新日期：2007-07-20 00:00:00

abstract：:Diversity of scaffold structure and function is a hallmark of the >50,000 isoprenoid natural products such as taxol. Whereas most members of this class are assembled by iterative head-to-tail enzymatic joining reactions between delta2- and delta3-isopentenyl diphosphate (IPP) monomers, dimerization of two delta2-IPP m...

journal_title：ACS chemical biology

authors： Walsh CT

更新日期：2007-05-22 00:00:00

abstract：:Methods for the rapid identification of defined cell growth conditions are lacking. This deficiency is a major barrier to the investigation and application of human embryonic stem (ES) cells. To address this problem, we developed a method for generating arrays of self-assembled monolayers (SAMs) in which each element ...

journal_title：ACS chemical biology

authors： Derda R,Li L,Orner BP,Lewis RL,Thomson JA,Kiessling LL

更新日期：2007-05-22 00:00:00

abstract：:Invertebrate animal models (mainly the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster) are gaining momentum as screening tools in drug discovery. These organisms combine genetic amenability, low cost, and culture conditions compatible with large-scale screens. Their main advantage is to allo...

journal_title：ACS chemical biology

authors： Ségalat L

更新日期：2007-04-24 00:00:00

abstract：:Angiogenesis, the formation of new blood vessels, is implicated in a number of important human diseases, including cancer, diabetic retinopathy, and rheumatoid arthritis. To identify clinically useful angiogenesis inhibitors, we assembled and screened a library of mostly Food and Drug Administration-approved drugs for...

journal_title：ACS chemical biology

authors： Chong CR,Xu J,Lu J,Bhat S,Sullivan DJ Jr,Liu JO

更新日期：2007-04-24 00:00:00

abstract：:This report highlights the advantages of low-affinity, multivalent interactions to recognize one cell type over another. Our goal was to devise a strategy to mediate selective killing of tumor cells, which are often distinguished from normal cells by their higher levels of particular cell surface receptors. To test wh...

journal_title：ACS chemical biology

authors： Carlson CB,Mowery P,Owen RM,Dykhuizen EC,Kiessling LL

更新日期：2007-02-20 00:00:00

abstract：:Cell-permeable small molecules can be used to modulate protein function selectively, rapidly, reversibly, and conditionally with temporal and quantitative control in biological systems. The identification of these chemical probes can require the screening of large numbers of small molecules. With the advent of new tec...

journal_title：ACS chemical biology

authors： Nicholson RL,Welch M,Ladlow M,Spring DR

更新日期：2007-01-23 00:00:00

abstract：:Engineering gene circuits with novel functions holds promise for broad applications in biology, engineering, and medicine. Directed evolution complements rational design as an important strategy for optimizing gene circuits and circuit elements. ...

journal_title：ACS chemical biology

authors： Song H,You L

更新日期：2006-12-20 00:00:00

abstract：:Modern visualization techniques are affording a peek into complex cellular processes. A recent paper describes an automated fluorescence microscopy method to map the subcellular localization of up to 100 different proteins in the same sample. ...

journal_title：ACS chemical biology

authors： Jacquier V,Michnick SW

更新日期：2006-12-15 00:00:00

abstract：:Detection of metabolites and post-translational modifications can be achieved using the azide as a bioorthogonal chemical reporter. Once introduced into target biomolecules, either metabolically or through chemical modification, the azide can be tagged with probes using one of three highly selective reactions: the Sta...

journal_title：ACS chemical biology

authors： Agard NJ,Baskin JM,Prescher JA,Lo A,Bertozzi CR

更新日期：2006-11-21 00:00:00

abstract：:The oxidative addition of nitric oxide (NO) to a thiol, S-nitrosation, is a focus of studies on cyclic guanosine monophosphate (cGMP)-independent NO signaling. S-Nitrosation of the catalytic cysteine of the caspase proteases has important effects on apoptosis and consequently has received attention. Here we report on ...

journal_title：ACS chemical biology

authors： Mitchell DA,Morton SU,Marletta MA

更新日期：2006-11-21 00:00:00

abstract：:The 3D structure of a protein is determined by the unique sequence of amino acid residues comprising the polypeptide chain. Sequence changes can cause protein misfolding, a potentially toxic phenomenon implicated in various neurodegenerative disorders. In a recent paper, translational misincorporation is proposed to b...

journal_title：ACS chemical biology

authors： Rochet JC

更新日期：2006-10-24 00:00:00

abstract：:Designed second mitochondrial activator of caspases (Smac) mimetics based on an accessible [7,5]-bicyclic scaffold bind to and antagonize protein interactions involving the inhibitor of apoptosis (IAP) proteins, X-chromosome-linked IAP (XIAP), melanoma IAP (ML-IAP), and c-IAPs 1 and 2 (cIAP1 and cIAP2). The design rat...

journal_title：ACS chemical biology

authors： Zobel K,Wang L,Varfolomeev E,Franklin MC,Elliott LO,Wallweber HJ,Okawa DC,Flygare JA,Vucic D,Fairbrother WJ,Deshayes K

更新日期：2006-09-19 00:00:00

abstract：:In a process called quorum sensing, bacteria produce and secrete certain signaling compounds (called autoinducers) that bind to receptors on other bacteria and activate transcription of certain genes. A clever genetic selection yields a new quorum-sensing transcriptional regulator that marches to the beat of a differe...

journal_title：ACS chemical biology

authors： Winans SC

更新日期：2006-08-22 00:00:00

abstract：:Pathogenic Yersinia spp. secrete the effector YopJ (YopP) into host cells to counteract cytokine production and to induce programmed cell death (apoptosis). YopJ achieves these aims by inactivating mitogen-activated protein kinase (MAPK) and nuclear factor kappaB signaling pathways. YopJ was shown to bind to members o...

journal_title：ACS chemical biology

authors： Bliska JB

更新日期：2006-07-21 00:00:00

abstract：:The degree to which anticancer agents selectively target cancer cells is a key determinant in successful therapeutic outcomes. Inhibitors of the Hsp90 molecular chaperone represent an important new class of anticancer agents. We propose here a novel mechanism by which physiochemical properties of Hsp90 inhibitors can ...

journal_title：ACS chemical biology

authors： Duvvuri M,Konkar S,Hong KH,Blagg BS,Krise JP

更新日期：2006-06-20 00:00:00

abstract：:Whereas the C. elegans genome was sequenced many years ago, the role of small molecule signals in its biology is still poorly understood. A recent publication reports the identification of two steroidal signaling molecules that regulate C. elegans reproductive development and dauer diapause via the nuclear receptor DA...

journal_title：ACS chemical biology

authors： Schroeder FC

更新日期：2006-05-23 00:00:00

abstract：:Recent studies have shown that cholesterol plays a significant role in the ability of Toxin A from Clostridium difficile to enter eukaryotic cells. The translocation process is one of three major steps during intoxication that could be targeted for intervention against the severe antibiotic-associated diarrhea caused ...

journal_title：ACS chemical biology

authors： Kerzmann A,Feig AL

更新日期：2006-04-25 00:00:00

abstract：:Zinc plays both physiological and pathological roles in biology, making it of increasing interest. To date, intracellular free zinc has been measured in cell types supplemented with or enriched in zinc, such as hippocampal neurons. Here we quantitatively image intracellular exchangeable zinc in an ordinary resting cel...

journal_title：ACS chemical biology

authors： Bozym RA,Thompson RB,Stoddard AK,Fierke CA

更新日期：2006-03-17 00:00:00